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. 2019 Apr 11;9:5917. doi: 10.1038/s41598-019-42253-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The effect of c-di-AMP and R848 on CTL responses as analysed by multicolour flow cytometry. (a–c) Percentages of IFN-γ⁺ (or TNF⁺, CD107a⁺) CD8⁺ T cells in the total memory CD8⁺ T cell population are plotted. (d,f) Percentages of SIV Gag-specific IFN-γ⁺ (or TNF⁺, CD107a⁺) CD8⁺ T cells among total memory CD8⁺ T cells. (g) Ratio of polyfunctional cells in SIV Gag-specific CD8⁺ T cells. Statistical significances were indicated by stars based on p-values (*P < 0.05, **P < 0.01). Horizontal bars indicate median levels. (h) The pie charts show the fraction of cells with one, two, and three functions. Statistical significances were indicated by permutation p-values.