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. 2019 Apr 11;10:1688. doi: 10.1038/s41467-019-09390-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Adsorption and DNA replication of the SNJ1 virus in CJ7-F cells expressing or lacking DndCDEA-PbeABCD. a Adsorption of SNJ1 to dndCDEA-pbeABCD-containing CJ7-F cells (red) is not impaired compared to that observed for CJ7-F cells containing the empty vector pFJ6-H (black). After the infection of logarithmic-stage cultures (OD600 = 0.3) with SNJ1 at an MOI = 1, samples were collected at 60-min intervals, and the extracellular (unabsorbed) viral concentration was measured and compared to the initial viral concentrations. The bars represent the means of three experiments, and the error bars represent the SD of the mean. b Real-time qPCR analysis to determine the replication efficiency of the virus SNJ1 in CJ7-F cells expressing (red) or lacking (black) dndCDEA-pbeABCD. Primers amplifying repA and radA were used to determine the concentration of SNJ1 DNA and CJ7-F chromosomes, respectively. The 2^C_T^(radA)– ^C_T^(repA) method was used to determine the relative levels of SNJ1 DNA in CJ7-F hosts. c Southern blot analysis of the SNJ1 genome during the infection cycle. Numbers indicate the time (min) following infection. A probe was designed corresponding to positions 9243–9732 nt in the SNJ1 genome. Source data are provided as a Source Data file