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. 2019 Apr 5;10:660. doi: 10.3389/fimmu.2019.00660

Figure 2.

Figure 2

5′MTPX library preparation. (A) Position of the 5′MTPX primers in the V gene leader sequence. (B) Schematic of the steps required to generate a library product using the 5′MTPX protocol, including cDNA synthesis, cDNA amplification using 5′MTPX primers and addition of Illumina adaptors. (C) Schematic of the 5′ and 3′ termini of a final library product produced using the 5′MTPX protocol. (D) A representative 1% agarose gel comparing DNA products obtained by 5′MTPX amplification of human IgM, IgG, IgK, and IgL cDNA.