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. 2019 Apr 5;10:713. doi: 10.3389/fimmu.2019.00713

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Copyright © 2019 Wendler, Schlundt, Bucher, Birkigt, Schipp, Volk, Duda and Schmidt-Bleek.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Histological analysis of in vivo experiments in mouse osteotomies at days 3 and 21 post-osteotomy. (A) Representative Movat's pentachrome pictures are displayed for the respective time point and group. (B–E) Histomorphometrical analysis of the Movat Pentachrome stainings: (B) bone marrow, (C) cartilage tissue, (D) connective tissue, and (E) mineralized bone tissue. Color coding of the Movat Pentachrome staining: mineralized bone = yellow, cartilage = dark blue-green, connective tissue = light blue-green, muscle fibers = light orange, cell nuclei = purple, fibrin clot = red/orange. Scale bars equal 500 μm, n = 6.