Figure 5.

Effect of Iloprost on mineralization capacity of osteogenically induced MSCs. Monolayers of MSCs were stained with Alizarin Red to visualize the formation of mineralized matrix. (A,B) Representative images of the Alizarin Red staining of cultured MSCs with osteoinductive media after 14 days of cultivation. Different concentrations of Iloprost were supplemented to the osteoinductive media (300 nM and 3 μM, respectively) (A). Quantification of the Alizarin Red staining (B). The metabolic activity of the cultured MSCs was measured by Prestoblue (C). Determination of the cell number of the cultured MSCs under the different stimuli (D). Scale bars: 200 μm; n = 6.