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. 2019 Apr 5;10:713. doi: 10.3389/fimmu.2019.00713

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Copyright © 2019 Wendler, Schlundt, Bucher, Birkigt, Schipp, Volk, Duda and Schmidt-Bleek.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Osteoimmunological effect of Iloprost treated bone marrow cells. α-CD3/α-CD28 activated bone marrow cells were cultured either with Iloprost or PBS as control. Obtained conditioned media was added to an osteogenic differentiation culture of MSCs and the secretion of mineralized matrix was measured by Alizarin Red staining. (A) Representative images of the Alizarin Red staining of the different culture conditions. (B) Quantification of the Alizarin Red signal. OM, osteoinductive media. Scale bars: 200 μm; n = 6.