Table 1.
Designation | DNA sequence (5′ to 3′) |
---|---|
Capture oligonucleotides | |
Capture Oligonucleotides1 | NH2-TTTTTTTTTTTTTTT ATTCCGCCGTGTATCGTAATTGAGT |
Capture Oligonucleotides2 | HS-TTTTTTTTTTTTTTTATTCCGCCGTGTATCGTAATTGAGT |
Capture Oligonucleotides3 | ACAGTACCGCAGGAAACGTTGTTTTTTTTTTTTTTT3′-SH |
Long oligonucleotides | |
Long Oligonucleotides1 | GAAGAGATTTTAGCGCAGTGTAGCATTACTGGATACTGCGATTATTGAACTCAATTACGATACACGGCGGAAT |
Long Oligonucleotides2 | GAAGAGATTTTAGCGCAGTGTAGCATTACTGGATACTGCGATTATTGAACTCAATTACGATACACGGCGGAATTTTTTTTTTTTTTTT-SH |
Primer pairs | |
Lolig-f/r | GAAGAGATTTTAGCGCAGTGTAG; CATTACTGGATACTGCGATTATTGA |
InvA-f/r | ACAGTGCTCGTTTACGACC; ACTGGTACTGATCGATAAT |