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. 2019 Apr 5;10:623. doi: 10.3389/fmicb.2019.00623

Figure 4.

Figure 4

YpsA inhibits FtsZ ring assembly. (A) Fluorescence micrographs of cells that either constitutively produce FtsZ-GFP in otherwise wild type strain (PE92; top panel) and cells that constitutively produce FtsZ-GFP and additionally harbor a copy of inducible ypsA (RB15) grown in the absence (middle panel) or presence of inducer IPTG are shown. Fluorescence of FM4-64 membrane dye (red) and GFP (green) are shown. (B) Cellular morphologies of cells that constitutively produce FtsZ-GFP (PE92) and cells that additionally contain a copy of inducible ypsA-mCherry (RB97) grown in the absence (middle panel) or presence of inducer are shown. DIC (gray) and fluorescence of GFP (green) and mCherry (red) are shown. (C) Cells of GG82 (inducible ypsA) and RB15 (inducible ypsA + constitutively expressed ftsZ-gfp) were imaged after grown in the presence of inducer for 1 h (top panels) or 3 h after the removal of inducer (bottom panels). Scale bars: 1 μm.