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. 2019 Apr 5;10:428. doi: 10.3389/fpls.2019.00428

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Copyright © 2019 Adam, Aviv-Sharon, Keren-Paz, Naveh, Rozenberg, Savidor and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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FTSH11 is located in the chloroplast envelope. Different extracts, organelles and fractions, from transgenic plants expressing FTSH11-HA, were subjected to imunoblot analysis with HA and control antibodies. (A) Total leaf and isolated chloroplast extracts. (B) Isolated mitochondria and chloroplasts. (C) Chloroplasts fractionated to membrane and soluble fractions. (D) Thylakoid and envelope membranes isolated on the same sucrose gradient. Marker proteins for the different compartments are: OE33 for thylakoids, ClpX for mitochondria, RbcL for stroma and OEP24 for envelopes. Antibodies used are indicated on the left and migration of MW markers (in kDa) are indicated on the right of each blot.