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. 2019 Apr 5;10:428. doi: 10.3389/fpls.2019.00428

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Copyright © 2019 Adam, Aviv-Sharon, Keren-Paz, Naveh, Rozenberg, Savidor and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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FTSH11-HA associates with CPN60. Total protein extracts from WT and EQ plants were solubilized with β-dodecyl maltoside, loaded on an immobilized anti-HA columns, thoroughly washed, and eluted with HA peptide. Samples from the different steps of the purification procedure were analyzed. (A) Immuno-blot analysis with HA, CPN60, and OE33 antibodies. Antibodies used are indicated on the left and migration of MW markers (in kDa) are indicated on the right of each blot. The genotypes of the samples are denoted on the far right of the blots. (B) Summary of MS analysis of the eluted fraction. The area of each section in the chart represents the abundance of the identified proteins within the eluted fraction from the EQ plants.