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. 2019 Apr 5;10:428. doi: 10.3389/fpls.2019.00428

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Copyright © 2019 Adam, Aviv-Sharon, Keren-Paz, Naveh, Rozenberg, Savidor and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Affinity enrichment of FTSH11-HA. Total protein extracts from EQ and KO plants were solubilized with β-dodecyl maltoside, incubated with immobilized anti-HA beads, and slightly washed. Proteins associated with the beads were then subjected to MS analysis. Volcano plots are presented. Significantly enriched chloroplast proteins interacting with FTSH11 are depicted in red. (De-enriched proteins are in blue, and proteins that were not significantly changed are in gray.) (A) Samples from plants grown at 20°C. (B) Samples from plants grown at 20°C and then exposed to 30°C for 6 h.