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. 2019 Apr 5;10:308. doi: 10.3389/fgene.2019.00308

FIGURE 3.

FIGURE 3

661W cells express markers of ciliated cell fate. (A) Heat map showing expression level of 33 Syscilia Gold Standard (SCGS) genes expressed in 661W cells which are either not expressed in hTERT-RPE1 cells, or are expressed at a low level in hTERT-RPE1 cells. Green dots indicate Joubert syndrome disease genes, blue dots indicate retinitis pigmentosa disease genes, orange dots indicate Bardet-Biedl syndrome disease genes, borwon dot indicates Senior-Loken syndrome disease gene, pink dot indicates Leber congenital amaurosis disease gene, yellow dot indicates Usher syndrome disease gene. Arrows indicate syndromic disease genes which are expressed in 661W cells but not expressed at all in hTERT-RPE1 cells. (B) Graph showing mean TPKM of selected SCGS genes in unstarved and serum starved cells, to show upregulation of these genes upon serum starvation to induce cilium formation. ∗∗∗p < 0.005; ∗∗p < 0.01; ∗p < 0.05.