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. 2019 Apr 5;10:308. doi: 10.3389/fgene.2019.00308

FIGURE 4.

FIGURE 4

661W cells grow long primary cilia. (A) 661W stained with proximal axonemal marker polyglutamylated tubulin (red), basal body marker γ tubulin (green) show that many cells grow primary cilia. Cells counterstained with DAPI (blue). Scale bar 10 μm. (B) Staining with cilium membrane marker Arl13b (green) shows that these cells grow cilia up to approx. 15 μm long, with only the proximal portion of the axoneme polyglutamylated. Scale bar 10 μm (C) Staining with Ift88 antibody (green) shows that this IFT protein localizes along the cilium, with more concentrated localisation at the base and tip of the cilium. Scale bar 10 μm. (D) Staining with Rpgrip1l antibody (green) shows that this transition protein colocalises with polyglutamylated tubulin, with more concentrated localisation at the base of this region of polyglutamylated tubulin. Scale bar 10 μm. (E) Scanning electron microscope image of 661 W cell, showing cilium in ciliary pit. Scale bar = 500 nm.