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. 2019 Apr 5;10:308. doi: 10.3389/fgene.2019.00308

FIGURE 5.

FIGURE 5

Ultrastructure of the 661 W cilium. (A) Hyvolution imaging of distal centriolar appendage marker Cep164 (red), which localizes specifically to the mature mother centriole of the basal body, and cilium membrane protein Arl13b (green). (B) Hyvolution imaging of distal centriolar appendage marker Cep164 (red), which localizes specifically to the mature mother centriole of the basal body, and γ tubulin (green) which labels both mother and daughter centrioles. Scale bar = 5 μm. (C) Hyvolution imaging of distal centriolar appendage marker Cep164 (red), and transition zone protein Rpgrip1l (green). Scale bar = 5 μm. (D) Schematic representation of protein localisation in 661W cilia, with scale bars, labeled. Asterisks symbolize region of polyglutamylation of the axoneme.