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. 2019 Apr 5;10:650. doi: 10.3389/fimmu.2019.00650

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Copyright © 2019 Kotlan, Horvath, Eles, Plotar, Naszados, Czirbesz, Blank, Farkas, Toth, Tovari, Szekacs, Shoenfeld, Godeny, Kasler and Liszkay.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Methodology flow chart on the selection of tumor binder scFv fragments by ELISA. The flow chart presents the new tumor infiltrating B cell antibody fragment phage display technology. It describes the biotechnological processes and our detection system. A master plate was prepared that enabled the detection of our scFvK phage display library in a soluble scFv and a phage displayed scFv form after rounds of panning reactions. Antibody fragments could be selected in the course of an ELISA using 95 well maxisorbe plates precoated with native cancer membrane preparations.