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. 2019 Apr 5;10:650. doi: 10.3389/fimmu.2019.00650

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Copyright © 2019 Kotlan, Horvath, Eles, Plotar, Naszados, Czirbesz, Blank, Farkas, Toth, Tovari, Szekacs, Shoenfeld, Godeny, Kasler and Liszkay.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Detection of GD3 ganglioside positive cancerous cells by immunofluorescence FACS analysis. Indirect immunofluorescence assay and FACS analysis showed that tumor-associated disialylated glycosphingolipid specific antibodies reacted intensively with primary cancerous cell outgrowth from metastatic melanoma (A), The majority (90%) of this cell population was GD3 ganglioside positive. Mean fluorescence intensity was about 100 times higher than the value of the negative control (B). IF FACS analysis proved strong binding capacity of our scFv anti GD3 antibody fragments against the cultured A2058 melanoma cells (C,D).