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. 2019 Apr 5;10:315. doi: 10.3389/fgene.2019.00315

FIGURE 5.

FIGURE 5

CDK12 is a direct target of miR-30c. (A) 3′-UTR analysis of CDK12 containing putative regions that match the seed sequence of miR-30c. The mutated nucleotides are underlined. (B) Overexpression of miR-30c inhibited the Renilla luciferase activities. HEK-293T cells were cotransfected with 5 nM miR-30c mimics and 500 ng of reporter plasmid containing the WT or MUT-type UTRs. 24 h later, Renilla luciferase values were normalized against firefly luciferase and presented. (C,D) miR-30c mimics or inhibitor were transfected into MDBKs. After 48 or 24 h, cells were harvested for western blot or RT-qPCR. (E,F) Embryos were treated with miR-30c mimics or control mimics and the relative levels of CDK12 was detected using RT-qPCR and WB. Data are presented as mean ± SD of three experiments. (∗∗P < 0.01).