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Chemical (un)folding studies of β‐cardiotoxin using guanidine hydrochloride. (a) Effect of guanidine HCl on secondary structure of β‐cardiotoxin. Chemical denaturation experiments were performed by dissolving β‐cardiotoxin in 25 mM Tris–HCL buffer pH 7.5 containing increasing concentrations of guanidine HCl. (b) Refolding of β‐cardiotoxin after the removal of guanidine HCl.
