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Chemical (un)folding studies of β‐cardiotoxin at different pH. (a) Effect of pH on secondary structure of β‐cardiotoxin. Chemical denaturation experiments were performed at 25°C by dissolving β‐cardiotoxin in buffers of different pH ranging from 2.5 to 9.5; 25 mM citrate pH 2.5, 25 mM MES pH 5.5, 25 mM MOPS pH 7.5, 25 mM Bis‐Tris propane pH 9.5.
