Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Combined effect of temperature and pH on the secondary structure of β‐cardiotoxin. Thermal denaturation of β‐cardiotoxin at pH 1.5. The protein was dissolved in MilliQ water (0.5 mg/mL) and pH adjusted to 1.5 with HCl and far‐UV CD spectra (a) and near‐UV CD spectra (b) were recorded using a 0.1 cm path‐length cuvette. Refolding was observed at 5°C after cooling from 85°C, black dotted line. The blue and black arrows indicate the new bands arising at 203 and 222 nm.
