Figure 1.

Variation of nanodisc lipid composition and its effects on CYP2J2 substrate metabolism. (A) CYP2J2 and CPR docked in nanodiscs of varying lipid compositions. Gray = POPC; blue = POPS; yellow = cholesterol; green = MSP. CYP2J2 is docked in the nanodisc via its N‐terminus and F‐G loop. CPR is added to solution separately and self‐associates with the lipid bilayer. (B) Structures of ebastine and its metabolites. Measurement of rate of NADPH oxidation and rate of metabolite formation from ebastine metabolism by CYP2J2‐CPR‐nanodiscs with varied lipid compositions. Inset shows carebastine production in SM containing nanodiscs. Rates are all calculated from within the initial, linear range. All metabolism data is compared to 20% POPS. * = P < 0.05; ** = P < 0.01; *** = P < 0.005. SEM is calculated from multiple measurements.