Figure 3.

Protection of LDH activity against desiccation‐induced inactivation. Buffered LDH (0.10 g/L) was desiccated and rehydrated by itself and with additives. Percent activity was determined by comparison to a sample of the same solution stored at 4°C. Uncertainties are the standard deviations of the mean from triplicate measurements. Data for the less effective additives sucrose, maltose, and mannitol are given in Supporting Information Figure S1. “LDH control” represents the activity in the absence of additive or in the presence of glucose, glycine, betaine or PEG 3350 (i.e., these additives are ineffective). Sigmoidal curves were added as a visual guide and used to calculate the concentration of additive required for 50% protection (Fig. 4). Data for trehalose and BSA have been published.16 Data for CAHS D and G are slightly different from our previous report16 because, as described in the Materials and Methods section, we improved the purification protocol.