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. 2019 Apr 5;10:323. doi: 10.3389/fgene.2019.00323

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Copyright © 2019 Li, Rao and Khanna.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of the Rp2-DKO mice. (A,B) Retinal extracts (100 μg) from the mice of the indicated genotypes were analyzed by SDS-PAGE and immunoblotting using anti-RP2, rhodopsin, S-opsin, cone PDE6α, and β-actin (loading control) antibodies. Fundus (C) and flat mounted retinal staining (D) of the Nrl^-/- and Rp2-DKO mice was performed. Arrows point to the white spots in the fundus photograph that correspond to the whorls and rosettes observed in the PNA (red; peanut agglutinin) stained flat mounted retinas of the Nrl^-/- mice. Such spots are undetectable in the Rp2-DKO mouse retinas.