TABLE 1.

Genomic features of metagenome-associated genomes^a

Genome feature	Data for strain:
	“Candidatus Colwellia aromaticivorans” sp. nov.	“Candidatus Halocynthiibacter alkanivorans” sp. nov.	“Candidatus Ulvibacter alkanivorans” sp. nov.
Coverage (×)	97	19	19
Completeness (%)	97.4	98.7	93.1
Contamination (%)	1.8	2.15	0.2
Size (Mbp)	3.71	4.7	2.7
No. of contigs	101	171	117
N50 value (bp)	72,862	55,069	47,750
No. of ORFsb	3,278	4,540	2,625
16S rRNA similarity with closest relative (%)	Colwellia rossensis S51-W(gv)1T (98.5)	Halocynthiibacter arcticus PAMC 20958T (97.2)	Ulvibacter antarcticus IMCC3101T (96.2)
GGDc with closest relative (%)	Colwellia rossensis S51-W(gv)1T (ND)d	Halocynthiibacter arcticus PAMC 20958T (19.4)	Ulvibacter antarcticus DSM 23424 (17.8)
AAI with closest relative (%)	Colwellia rossensis S51-W(gv)1T (ND)	Halocynthiibacter arcticus PAMC 20958T (65.4)	Ulvibacter antarcticus DSM 23424 (71.8)

^a16S rRNA sequences recovered were retrieved with RNAmmer (14) and compared with BLAST (15) against the Silva database (16, 17). The closest were selected to align through ssu-align v0.1.1 (18, 19) and perform phylogeny through IQ-TREE (20–22). The 16S rRNA similarity was calculated with TaxonDC (23). AAI and DDH values were performed with CompareM (https://github.com/dparks1134/CompareM) and GGDC (http://ggdc.dsmz.de/), respectively. The genome of Ulvibacter antarcticus DSM 23424 was used for AAI and DDH analysis. In silico-predicted phenotype analysis reveals that “Candidatus Colwellia aromaticivorans” sp. nov. is positive for chitin hydrolysis, propionate utilization, and dl-lactate utilization. “Candidatus Halocynthiibacter alkanivorans” sp. nov. is positive for acid production from d-ribose, sucrose, and glycerol; acetate utilization; indole production; and nitrate reduction. “Candidatus Ulvibacter alkanivorans” sp. nov. is positive for dl-lactic acid utilization.

^bORFs, open reading frames.

^cGGD, genome-to-genome distance.

^dND, not determined.