Figure 7.

Effects of Ajuga sp. extracts on the oxidative stress generated by C26 colon carcinoma cells. (A,B) Malondialdehyde (MDA) concentration after (A): IC₅₀ EEAG, IC₅₀ EEAC, and IC₅₀ EEAL treatment and (B): IC₈₀ EEAG, IC₈₀ EEAC, and IC₈₀ EEAL treatment. (C,D) Catalytic activity of catalase after (C): IC₅₀ EEAG, IC₅₀ EEAC, and IC₅₀ EEAL treatment, and (D): IC₈₀ EEAG, IC₈₀ EEAC, and IC₈₀ EEAL treatment. (E,F) Total non-enzymatic antioxidant system levels in the cell lysates obtained from standard culture of C26 colon carcinoma cells after 24 h of incubation with (E): IC₅₀ EEAG, IC₅₀ EEAC, and IC₅₀ EEAL treatment, and (F): IC₈₀ EEAG, IC₈₀ EEAC, and IC₈₀ EEAL treatment. One way ANOVA test with Bonferroni correction for multiple comparisons was performed to analyze the differences between the effects of the treatments applied on MDA and non-enzymatic antioxidant defense systems levels and on catalase activity. Control, untreated C26 cells; IC₈₀ or IC₅₀ EEAG, cells incubated with 650 μg/mL or 457.5 μg/mL A. genevensis ethanolic extract; IC₈₀ or IC₅₀ EEAC, cells incubated with 650 μg/mL or 303 μg/mL A. chamaepitys ethanolic extract; IC₈₀ or IC₅₀ EEAL, cells incubated with 325 μg/mL or 176.3 μg/mL A. laxmannii ethanolic extract. (ns, P > 0.05; ^∗P < 0.05; ^∗∗∗P < 0.001).