Figure 8.

Effects of Ajuga sp. extracts on the oxidative stress generated by B16.F10 melanoma cells. (A,B) MDA concentration after (A): IC₂₀ EEAG, IC₅₀ EEAC, and IC₅₀ EEAL treatment and (B): IC₄₀ EEAG, IC₈₀ EEAC, and IC₈₀ EEAL treatment. (C,D) Catalytic activity of catalase after (C) IC₂₀ EEAG, IC₅₀ EEAC, and IC₅₀ EEAL treatment and (D): IC₄₀ EEAG, IC₈₀ EEAC, and IC₈₀ EEAL treatment. (E,F) Total non-enzymatic antioxidant system levels in the cell lysates obtained from standard culture of B16.F10 murine melanoma cells after 24 h of incubation with (E): IC₂₀ EEAG, IC₅₀ EEAC, and IC₅₀ EEAL treatment and (B): IC₄₀ EEAG, IC₈₀ EEAC, and IC₈₀ EEAL treatment. One way ANOVA test with Bonferroni correction for multiple comparisons was performed to analyze the differences between the effects of the treatments applied on MDA and non-enzymatic antioxidant defense systems levels and on catalase activity. Control, untreated B16.F10 cells; IC₄₀ or IC₂₀ EEAG, cells incubated with 650 μg/mL or 260 μg/mL A. genevensis ethanolic extract; IC₈₀ or IC₅₀ EEAC, cells incubated with 650 μg/mL or 406.7 μg/mL A. chamaepitys ethanolic extract; IC₈₀ or IC₅₀ EEAL, cells incubated with 325 μg/mL or 236.8 μg/mL A. laxmannii ethanolic extract. (ns, P > 0.05; ^∗P < 0.05; ^∗∗P < 0.01; ^∗∗∗P < 0.001).