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. 2019 Apr 5;10:704. doi: 10.3389/fmicb.2019.00704

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Copyright © 2019 Wang, Guan, Zhang, Chen and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SDS-PAGE of the recombinant PB2SF from E. coli. PB2SF was purified through Ni²⁺-chelation chromatography and gel-filtration chromatography. Enzyme purity following each fractionation step was assessed by SDS-PAGE using 13.2% polyacrylamide gels, followed by staining with Coomassie brilliant blue. Lane 1, unstained protein molecular weight marker SM 0431 (Thermo); Lane 2, uninduced cell lysate; Lane 3, induced cell lysate; Lane 4, supernatant of the induced cell lysate; Lane 5, purified recombinant PB2SF.