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. 2019 Apr 5;10:704. doi: 10.3389/fmicb.2019.00704

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Copyright © 2019 Wang, Guan, Zhang, Chen and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of the final products of unsaturated GAG disaccharides treated with PB2SF. Unsaturated CS/DS disaccharides ΔC (A), ΔA (B), ΔD (C), and ΔT (D) and unsaturated Hep/HS disaccharides (E) were incubated without (top) or with (bottom) PB2SF, labeled with 2-AB and then analyzed via anion-exchange HPLC, as described in Section “Materials and Methods.” In (A–D), the elution positions of the standard disaccharides are indicated: ΔO (Δ^4,5HexUAβ1-3GalNAc), ΔC [Δ^4,5HexUAβ1-3GalNAc(6S)], ΔA [Δ^4,5HexUAβ1-3GalNAc(4S)], ΔD [Δ^4,5HexUA(2S)β1-3GalNAc(6S)], ΔE [Δ^4,5HexUAβ1-3GalNAc(4S,6S)], and ΔT [Δ^4,5HexUA(2S)β1-3GalNAc(4S,6S)]. In (E), the elution positions of the following standard disaccharide components are indicated by numbers, (1) Δ^4,5HexUAα1-4GlcNAc(6S); (2) Δ^4,5HexUAα1-4GlcNS; (3) Δ^4,5HexUAα1-4GlcNS(6S); (4) Δ^4,5HexUA(2S)α1-4GlcNS; and (5) Δ^4,5HexUA(2S)α1-4GlcNS(6S).