Figure 5.

The number of Lcl collagenous repeats is correlated with biofilm production in L. pneumophila clinical isolates. (A) PCR amplification of lcl in the indicated L. pneumophila clinical isolates visualized by agarose gel electrophoresis. (B) Biofilm formation of 2 days old biofilms quantified by crystal violet of the indicated L. pneumophila clinical isolates. (C) Biofilm formation of 2 days old biofilms of L. pneumophila clinical isolates containing 18 and 13 repeats. Eight different strains were included per group and are indicated in Table 2. ^∗ and^∗∗ denotes p < 0.05 and p < 0.01, respectively, by one-way ANOVA.