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Relative gene expression levels of toll‐like receptors (TLRs) in tissues of chickens challenged with Newcastle disease virus (NDV) strain GD (Md/CH/LGD/1/2005,) and NDV strain F48E9 respectively. The cDNA copy numbers in the tissue samples were measured by quantitative RT‐PCR. The mRNA levels of target genes were normalized to that of 18S rRNA in the same samples. Each bar is the mean ± SD. a,bValues with superscripted different letters are significantly different (p < 0.05)
