Figure 5.

Nickel decreases levels of extracellular enterobactin and its hydrolysis products during lag phase (a) Spent media from Wild‐type MG1655 culture with 0 μM nickel or 50 μM NiCl₂ was filtered and enterobactin‐related metabolites were separated by FPLC (b) Spent media from ΔfepA cultures with no added nickel or 50 μM NiCl₂ was filtered and enterobactin‐related metabolites were separated by FPLC. Elution times are shown across the bottom axis. The elution peak annotated ‘1’ refers to the linear dimer, ‘2’ refers to the hydrolytically cleaved linear trimer, ‘3’ refers to the nonhydrolytically cleaved linear trimer, and ‘4’ refers to cyclized enterobactin