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. Author manuscript; available in PMC: 2019 Apr 12.
Published in final edited form as: Exp Cell Res. 2016 Jul 14;347(1):42–51. doi: 10.1016/j.yexcr.2016.07.005

Figure 2.

Figure 2

Rab3A distribution during egg activation. A) MII eggs were collected and activated with 10 mMSrCl2. After 0, 10, 20, and 40 min of activation, cells were fixed, permeabilized and immunostained with anti-Rab3A antibody (top row). Cortical granules were stained with LCA conjugated with rhodamine and DNA was stained with Hoechst 33342 (middle row). Bottom row shows merged images from top and middle row. Shown are representative images of three independent experiments. Scale bar, 50 μM. B) Immunoblot analysis of Rab3A protein in protein extracts derived from 100 non activated MII eggs (Ctrl) and 100 activated MII eggs (SrCl2). Proteins were resolved on 10 % SDS-PAGE gel and immunoblotted with anti-Rab3A as described in Materials and Methods. The experiment was performed 3 times and a representative blot is shown. C) Analysis of fluorescence-intensity distribution (measured in arbitrary intensity units, A.I.U.) of Rab3A and cortical granules (CG) for the MII egg showed in A. On the right, the graph also shows cortical granules density (CG/100 μm2) corresponding for each time of activation (for more details see text).