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. Author manuscript; available in PMC: 2019 Apr 12.
Published in final edited form as: Cell Metab. 2018 Jun 5;27(6):1222–1235.e6. doi: 10.1016/j.cmet.2018.05.006

Figure 5. Fecal microbiota transplantation (FMT) from mice on IF is protective in EAE.

Figure 5.

Mice were pre-treated with an antibiotic cocktail for a week, and then subjected to FMT from donor mice that were on IF (for 4 weeks) or fed ad libitum. FMT was administered for a week before and a week after EAE immunization. (A) EAE clinical course in mice transferred with fecal matters from mice on IF or fed ad libitum. Shown is one representative experiment out of two performed with similar results (each dot represent the mean clinical scores for all 5 mice in each group; error bars are SEM.; P<0.0001 by two-way ANOVA; Table S2 reports clinical characteristics for the two experiments performed). (B) Spinal cord pathology: the upper panel shows histological staining (solochrome cyanine) for myelin (in blue) and the lower panel shows immuno-staining for SMI-32+ damaged axons (in red) and MBP (in green). (C) Quantification of inflammation, demyelination (evaluated by histology and MBP staining) and axonal damage (evaluated by SMI-32+ staining) in the spinal cord in the two groups (n=10/group). Each dot represents a mouse and the bars are means ± SD.