KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Purified anti-Neurofilament H (NF-H), Nonphosphorylated Antibody, SMI-32 | Sternberger Monoclonals | Cat 801701 |
| Rat Anti-Myelin Basic Protein antibody | Abcam | Cat ab7349 |
| CD4 Brilliant Violet 421 | Biolegend | Clone: GK1.5 Cat 100437 |
| CD3 PE | Biolegend | Clone 17A2 Cat 100215 |
| CD62L AF700 | eBioscience | Clone MEL-14 Cat 56-0621-82 |
| CD25 AF647 | Biolegend | Clone PC61 Cat 102019 |
| CD44 APC | Biolegend | Clone IM7 Cat 103011 |
| CD8a AF488 | Biolegend | Clone 53–6.7 Cat 100726 |
| CD45 APC/Cy7 | Biolegend | Clone 30F11 Cat 103115 |
| FoxP3 FITC | eBioscience | Clone PCH101 Cat 11-4776-42 |
| IL-17A FITC | Biolegend | Clone TC11–18H10.1 Cat 506907 |
| IFN-γ APC | Biolegend | Clone XMG1.2 Cat 505809 |
| IL-10 PercP | Biolegend | Clone JES5–16E3 Cat 505027 |
| GM-CSF PE | Biolegend | Clone MP1–22E9 Cat 505405 |
| Zombie Acqua | Biolegend | Cat 423101 |
| Human CD303 (BDCA-2) FITC | Biolegend | Clone 201A Cat 354208 |
| Human CD304 (BDCA-4) PE | Biolegend | Clone 12C2 Cat 354504 |
| Human Lin-1 APC | eBioscience | Cat 22-7778-72 |
| Human CD86 AF700 | BD Biosciences | Clone FUN-1 Cat 561124 |
| Human Cd1c BV421 | Biolegend | Clone L161 Cat 331526 |
| Human HLA-DR Krome Orange | Beckman Coulter | Cat B00070 |
| Human CD196 (CCR6) Pe/Cy7 | eBioscience | Clone R6H1 Cat 25-1969-42 |
| Human CD56 PE | eBioscience | Cat 555516 |
| Human CD4 ECD | Beckman Coulter | Clone T4 Cat 6604727 |
| Human CD14 Pe/Cy7 | eBioscience | Clone 61D3 Cat 25-0149-42 |
| Human CD19 APC | Biolegend | Clone SJ25C1 Cat 363007 |
| Human CD8 Krome Orange | Beckman Coulter | Clone B9.11 Cat B00067 |
| Human CD39 FITC | BD Pharmigen | Clone TU66 Cat 561444 |
| Human CD25 PE | Biolegend | Clone M-A251 Cat 356104 |
| Human FoxP3 APC | Biolegend | Clone 259D Cat 320216 |
| Human CD45RA FITC | BD Pharmigen | Clone SK3 Cat 555488 |
| Human CD45RO PE | BD Pharmigen | Clone UCHL1 Cat 560607 |
| Human CD62L PercP710 | eBioscience | Clone DREG-56 Cat 46-0629-42 |
| Human CD197 (CCR7) AF647 | eBioscience | Clone 150503 Cat 581143 |
| 7-aminoactinomycin (7-AAD) | BD Pharmigen | Cat 559925 |
| Bacterial and Virus Strains | ||
| Mycobacterium tuberculosis (strain H37RA) | Fisher-scientific | Cat BD™ 231141 |
| Biological Samples | ||
| Human Whole blood | John L. Trotter MS Center | NCT02411838 |
| Stool samples of reference cohort used for 16S rDNA amplification and sequencing | John L. Trotter MS Center | NCT02411838 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| MOG aa 35–55 MEV GWY RSP FSR VVH LYR NGK | GenScript | ID: 126711–1 |
| Metronidazole 99% | ACROS Organics | Cat 443-48-1 |
| Vancomycin hydrochloride | Sigma-Aldrich | Cat 1404-93-9 |
| Neomycin trisulfate salt hydrate | Sigma-Aldrich | Cat N6386 |
| Ampicillin | Sigma-Aldrich | Cat A9393 |
| Phorbol 12-myristate 13-acetate (PMA) | Sigma-Aldrich | Cat P8139 |
| Ionomycin calcium salt from Streptomyces conglobatus | Sigma-Aldrich | Cat I0634 |
| Monensin | eBioscience | Cat 00-4505-51 |
| Pertussis Toxin from B. pertussis, Lyophilized in Buffer | List Biological laboratories, Inc | Cat 180 |
| Eriochrome cyanine R (C.I. 43820) | Sigma-Aldrich | Cat 1.03164 |
| Iron(III) chloride | Sigma-Aldrich | Cat 451649 |
| Hepes Buffer | Corning | Cat 25060CI |
| Fetal bovine serum, FBS | Gibco | Cat 2614–079 |
| EDTA 0.5 M | Corning | Cat 40–034Cl |
| Dithiothreitol (DTT) | Bio-Rad | Cat 1610610 |
| Percoll | GE Healthcare | Cat 17-0891-01 |
| Hank’s balanced salt solution (+) | Gibco | Cat 24020–117 |
| Hank’s balanced salt solution (−) | Gibco | Cat 14175–079 |
| Critical Commercial Assays | ||
| Lamina Propria Dissociation Kit, mouse | Miltenyi Biotec | Cat 130-097-410 |
| Corticosterone EIA Kit | Arbor Assays | Cat K014-H1 |
| Foxp3 Staining Buffer Set | eBioscience | Cat 00-5523-00 |
| Mouse Adiponectin ELISA | EMD Millipore | Cat Mill-EZMADP-60K |
| Mouse Leptin ELISA | EMD Millipore | Cat Mill-EZML-82K |
| β-Hydroxybutyrate (Ketone Body) Colorimetric Assay Kit | Cayman Chemical Company | Cat 700190 |
| Adiponectin Human ELISA, High Sensitivity (Sandwich) | BioVendor | Cat RD191023100 |
| Human Leptin “Dual Range” ELISA | EMD Millipore | Cat EZHL-80SK |
| Deposited Data | ||
| 16S and Shotgun microbiome data | SRA database | PRJNA388060 |
| Experimental Models: Organisms/Strains | ||
| C57BL/6, Black 6 | Jackson Laboratory | Cat 000664 |
| Software and Algorithms | ||
| Metamorph 7.7 software | Molecular Devices | ftp://ftp.meta.moleculardevices.com/pub/uic/software/CDROM/MM77.iso |
| Kaluza 1.3 | BeckmanCoulter | https://www.beckman.com/coulter-flow-cytometers/software/kaluza |
| GraphPad Prism, 7 | GraphPad Software | https://www.graphpad.com/scientific-software/prism/ |
| FlowJo_V10 | FlowJo, LLC | https://www.flowjo.com/ |
| R for microbiome analysis- R version 3.2.2 (2015-08-14) | R team | https://www.r-project.org/ |
| SAS v9.4 | SAS | https://www.sas.com/en_us/software/sas9.html |
| Other | ||
| Gentle MACS TM Dissociators | Miltenyi Biotec | Cat 130-096-427 |