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(A) Protein expression profiles of TLR4 (96 kD), NF-κB (65 kD), IκB-α (36 kD), NOX2 (60 kD) within the PVN by western blot. TLR4, IκB-α, NOX2 were quantified relative to the GAPDH (37 kD) levels (B to D), and NF-κB was quantified relative to the Histone 3 (15 kD) level (E). n = 8–9 per time-point. *P < 0.01 and **P < 0.05 versus control group.
