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(A) Representative protein expression levels of TLR4 (96 kD), NF-κB (65 kD), IκB-α (36 kD), and NOX2 (60 kD) as determined by western blot. The cytokine levels of IL-1β(F) and TNF-α(G) from the PVN tissue as measured by ELISA. n = 10–11 for each group. *P < 0.01 and **P < 0.05 versus sham groups; &P < 0.01 and #P < 0.05 versus MI + shCtrl group.
