Table 1.
Prevalence of enterotoxin and virulence genes, MLST and MALDI‐TOF MS for suspected Staphylococcus argenteus identification
| Isolate no. | Enterotoxin and virulence genes detection | MLST identification | Sequence results of NRPS PCR amplicon | MALDI‐TOF MS identification | |||
|---|---|---|---|---|---|---|---|
| ST | CC | Size (bp) | BLAST species | Sequence identities (%) | |||
| U27 OC2.1 | ND | ST4209a | Singleton | 160 | S. aureus strain K5 (CP020656.1) | 97 | S. aureus |
| U27 OC2.2 | ND | ST4209a | Singleton | 160 | S. aureus strain FDAARGOS (CP007539.3) | 99 | S. aureus |
| U27 OC2.3 | ND | ST4209a | Singleton | 160 | S. aureus strain K5 (CP020656.1) | 96 | S. aureus |
| U19 T10.1 | sed, hla, hlb, clfA, pvl | ST4210a, b | Singleton | 340 | S. argenteus strain XNO106 (CP025023.1) | 99 | S. argenteus |
| U43 S18.1 | sea, hla, hlb, clfA | ST4211a, b | Singleton | 340 | S. argenteus strain XNO106 (CP025023.1) | 100 | S. argenteus |
| U43 S18.3 | sea, sec, sed, hla, hlb, clfA, pvl | ST4211a, b | Singleton | 340 | S. argenteus strain XNO106 (CP025023.1) | 100 | S. argenteus |
| U14 T6.2 | ND | ST4212a | Singleton | 160 | S. aureus strain FDAARGOS_159 (CP014064.2) | 95 | S. aureus |
| U65 S3 | ND | ST4213a | Singleton | 160 | S. aureus strain NRS137 (CP026080.1) | 92 | S. aureus |
bp: base pair; BLAST: basic local alignment search tool; CC: clonal complex; MALDI‐TOF MS: matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry; ND: not determine; MLST: multilocus sequence type; NRPS: nonribosomal peptide synthetase.
a
Novel STs from this study.
b
S. argenteus ST.