Figure 2. Reduced tumor cell proliferation and inflammation in Kras ^G12D:Adam17 ^ex/ex mouse lungs.

• A
  Representative images of PCNA‐stained lung sections from Kras ^WT, Kras ^G12D, and Kras ^G12D:Adam17 ^ex/ex mice at 6 weeks post‐Ad‐Cre (for Kras ^G12D) or vehicle (for Kras ^WT) inhalation. Scale bar, 100 μm.
• B
  Quantification of PCNA‐positive cells/high‐power field (HPF) in the indicated mouse lungs (n = 6 per genotype). **P < 0.01, Student's t‐test, mean ± SEM.
• C
  qPCR expression analyses of cell cycle regulation genes (normalized against 18SrRNA) in lungs from the indicated mice (n = 6 per genotype). *P < 0.05, Student's t‐test, mean ± SEM.
• D
  Representative immunoblots of Kras ^G12D and Kras ^G12D:Adam17 ^ex/ex lung lysates with the indicated antibodies. Each lane is an individual mouse sample.
• E
  Semi‐quantitative densitometry of Myc protein levels (relative to actin) in lung lysates from (D). n = 6 per genotype. **P < 0.01, Student's t‐test, mean ± SEM.
• F, G
  Representative images (F) and quantification of CD45‐positive cells/HPF (G) in CD45‐stained lung sections from the indicated genotypes at 6 weeks post‐inhalations, mean ± SEM. In (F), scale bar, 100 μm. In (G), n = 6 per genotype. **P < 0.01, Student's t‐test.

Data information: Exact P values are specified in Appendix Table S4.Source data are available online for this figure.