Figure 4. Acitretin increases APP but not NrCAM shedding in primary neurons and AD patients.
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AWt neurons were kept in culture until DIV5; then, the cells were treated with acitretin (4 μM) or vehicle control for 48 h, as described earlier (Tippmann et al, 2009).
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BDensitometric quantifications of the Western blots are shown (***P < 0.001; ****P < 0. 0001, two‐sided Student's t‐test, n = 9–20). The mean levels of solvent‐treated cells were set to 1.
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CDetection of sNrCAM and human serum albumin (hSA) in AD patients CSF that had been treated with acitretin (30 mg/day) or placebo for 30 days (n = 9) (Endres et al, 2014). b = baseline; t = treatment.
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DDensitometric quantification of the Western blots in (C). sNrCAM/hSA ratios were calculated, and then, the treatment‐to‐baseline (t/b) ratios for every patient were calculated. Two‐sided Student's t‐test (n = 9).