Figure EV5. Neuronal stimulation by NMDA increases ADAM10‐mediated NrCAM shedding.

• A
  To genetically validate our findings, ADAM10fl/fl neurons were treated with an iCre, or a control lentivirus at DIV2, to knock out ADAM10. The cells were kept in culture until DIV10; then, the neurons were treated with NMDA (50 μM) or vehicle for 30 min (Wan et al, 2012).
• B
  Wt neurons were cultured like in (Fig 5A). At DIV10, the cells were pretreated with the NMDA receptor antagonist D‐APV (100 μM) or vehicle for 30 min; then, the neurons were treated with NMDA (50 μM) or vehicle for 30 min. Densitometric quantifications of the Western blots are shown. One‐way ANOVA with post hoc Dunnett's test (****P < 0. 0001, n = 6). Shown are the mean and SEM. Representative Western blots are shown.

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