Fig. 1.

a Mouse Cnr1 (4A5) and b rat Cnr1 gene (5q21) structure and transcript variants. The open boxes represent the exons, with the exon number marked inside the box, and horizontal lines represent introns. A fused exon–intron is shown by an open box with a middle line. The intra-exonal introns are shown by smaller shaded boxes, and arrows represent intra-exonal splicing sites. The alternatively spliced transcript variants are shown under the genes, and GenBank accession numbers are included in the parenthesis. The TaqMan probes were designed to hybridize in junctions of spliced exons represented by thin lines under exon–exon junctions. RNAscope ZZ pair probes are marked as black bars under exon 1, retaining intron 1 and the 3′UTR of the mouse Cnr1 gene