Table 1.
TaqMan probe sequences for mCB1 and rCB1 isoforms (all the probes are FAM-labeled except endogenous controls of mActb and rGapdh are VIC-labeled)
| Gene | TaqMan probe | Forward primer | Reverse primer |
|---|---|---|---|
| mCB1A | TGCGAAGGGGTTCC | GTGCCGAGGGAGCTTCTG | CCGTCTAAGATCGACTTCATAACCT |
| mCB1B | ATGGCTGAGGGTTC | CCCGTTTCAAGCAAGGAACA | GGCTCAACGTGACTGAGAAAGA |
| mCB1C | CAGAAGTAAATGCTGTAGAAGA | GCACTTAGTAGGAGCAAACATCTAAGC | TCTAACATTGTATCGACTACTCTCTATGGTT |
| mCB1a | TCCTCTACGTGGGCTC | GATACCACCTTCCGTACCATCAC | AATGTTGGTTGTGTCTCCTTTGATAT |
| mCB1b | ACAGACCTCCTCTACGTGG | GATACCACCTTCCGTACCATCAC | GTTGTCCTCGTTCTCCTGAATGT |
| mCB1R | Mm01212171_s1 | (Taqman catalog number of ABI) | |
| mCB2A | CTGACAAATGACACCCAGTC | CAGGACAAGGCTCCACAAGAC | GATGGGCTTTGGCTTCTTCTAC |
| mCB2B | TGGGCCCAGTCTT | GCCACCCAGCAAACATCTCT | GATGGGCTTTGGCTTCTTCTAC |
| mActb | 4352341E | (Taqman catalog number of ABI) | |
| rCB1A | TGAGAAGGGGTTCC | GTGCCGAGGGAGCTTCTG | GACTCAAGGTGACTGAGAAAGA |
| rCB1B | ATGGCTGAGGGTTC | CCCATTTCAAGCAAGGAGCA | GACTCAAGGTGACTGAGAAAGA |
| rCB2A | CTGACAAATGACTCCCAGTC | CAGGACAAGGCTTCACAAGAC | GACAGGCTTTGGCTGCTTCTAC |
| rCB2B | TGGGCCCAGTCCT | GCCACCCAGCAAACATCTAT | GACAGGCTTTGGCTGCTTCTAC |
| rGapdh | CTCATGACCACAGTCCA | GACAACTTTGGCATCGTGGAA | CACAGTCTTCTGAGTGGCAGTGA |
| rNeuN | CACTCCAACAGCGTGAC | GGCCCCTGGCAGAAAGTAG | TTCCCCCTGGTCCTTCTGA |
| rGfap | Rn00574125_g1 | (Taqman catalog number of ABI) | |
| rIba-1 | Rn00566603_m1 | (Taqman catalog number of ABI) |