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. 2019 Apr 5;10:323. doi: 10.3389/fphar.2019.00323

FIGURE 1.

FIGURE 1

Propofol and lipid emulsion inhibit PMA-induced NET production. (A) PMA-induced NET production/extracellular DNA in the presence or absence of propofol/lipid emulsion was quantified using the fluorescent DNA stain PicoGreen (n = 4). Data were analyzed using one-way ANOVA (p = 0.0033) with Dunnett’s multiple comparisons test (p = 0.0086 PMA vs. PMA + propofol; p = 0.0153 PMA vs. PMA + lipid emulsion). (B) PMA-induced NET production with and without pre-treatment with propofol or lipid emulsion was visualized via fixation of neutrophils and staining using an antibody against myeloperoxidase (n = 3; representative image shown). Propofol concentration was 5 μg/mL for all experiments, with lipid emulsion concentration adjusted to match the soybean oil concentration in propofol.