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. 2019 Jan 21;29(3):183–192. doi: 10.1038/s41422-018-0131-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Rationally designed SpCas9 variants have higher specificity in human cells. a Analysis of the DNA targeting specificity of rationally designed SpCas9 variants in human cells. Frequencies of Indels induced by rationally designed SpCas9 variants guided by doubly mismatched sgRNAs against EMX1 site bearing 5′-TGG-3′ PAM quantified by T7E1 assay. n = 3. Error bars represent S.D. b Analysis of the DNA targeting specificity of rationally designed SpCas9 variants in human cells. Frequencies of Indels induced by rationally designed SpCas9 variants guided by doubly mismatched sgRNAs against EMX1 site bearing 5′-CGA-3′ PAM quantified by T7E1 assay. n = 3. Error bars represent S.D. c Analysis of the DNA targeting specificity of rationally designed SpCas9 variants in human cells. Frequencies of Indels induced by rationally designed SpCas9 variants guided by doubly mismatched sgRNAs against EMX1 site bearing 5′-CGT-3′ PAM quantified by T7E1 assay. n = 3. Error bars represent S.D