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. 2019 Apr 5;10:598. doi: 10.3389/fimmu.2019.00598

Figure 2.

Figure 2

The migratory population contains diverse cell types, but the antigen presenting cells within this population are predominantly CD103+ CD11b+ dendritic cells. Kaede mice were injected with Alum/LPS/Eα-OVA in the hind footpad, 12 h later the tissue was photo-converted and the animals were culled after a further 12 h. (A) Analysis of the sorted, migratory population is shown with cells expressing MHCII represented in red, innate cells in blue and T cells in green. (B–D) The spade diagrams show the levels of antigen presentation (YAe) detected on the lymph node resident population in untreated mice, mice treated with alum/LPS in the foot pad and the migratory population of mice treated with alum/LPS in the footpad. Red and yellow nodes represent high and low expression of Eα:MHCII complexes, respectively. (E) Dendritic cells were identified based on expression of CD11c and MHC class II. Representative dot plots identifying antigen presenting DCs and (F) expression of CD44 and CD86 are shown. (G) The phenotype of DCs that expressed Eα:MHCII complexes and those that did not were further investigated based on the expression of CD103 and CD11b. n = 3 ± 1 SD (****p < 0.0001).