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. 2019 Apr 5;10:598. doi: 10.3389/fimmu.2019.00598

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Copyright © 2019 Hayes, Rane, Scales, Meehan, Benson, Maroof, Schroeder, Tomura, Gozzard, Yates, Garside and Brewer.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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B1a, B1b, and B2 cells can be identified in the migratory population. Kaede mice were injected with alum/LPS or saline in the hind footpad and photo-conversion was performed 12 h later. Mice were culled after a further 12 h and the popliteal lymph nodes analyzed by flow cytometry. (A) The gating strategy used to identify B cells from the migratory population is highlight. Migratory cells were first identified and B cells selected based on B220 and CD19 expression. B cells subsets were identified using CD5 and CD43. We show (B) The proportion and (C) numbers of each B cell subset. Data are representative of two independent experiments. n = 3 ±1 SD.