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. 2018 Mar 19;16(3):275–287. doi: 10.1038/cmi.2018.5

Figure 4.

Figure 4

IL-2-induced pSTAT5 levels in T-cells from both study groups, and effects of lentiviral SOCS3 modulation on IL-2-induced pSTAT5. (a and b) IL-2-induced STAT5 phosphorylation of CD4+ T-cells is shown for tuberculosis patients (grey squares) and healthy contacts (open circles). (b) Correlation of IL-2-induced pSTAT5 with SOCS3 expression. The Spearman Rank test was used to determine significant correlations for all donors and both study groups separately. Correlation coefficients (rho) and nominal P-values are given. (c) Representative flow cytometry histogram indicating fluorescence (eBFP) expression of non-transduced, vector control-, SOCS3cDNA-, and SOCS3shRNA-transduced CD4+ T-cells are shown. (d) Flow cytometry-based quantification of CD4+ T-cells with SOCS3cDNA (dark grey) and SOCS3shRNA (black line, open) compared to vector control (bright grey). The results from seven experiments are shown. (e) STAT5 phosphorylation in CD4+ T-cells from three experiments with increased or reduced SOCS3 expression. Nominal P-values for the paired t Test (two-tailed) were shown. MFI, mean fluorecence intensity.