Table 1.
Primer sequences used in qPCR
| Target | Primer | Sequence |
|---|---|---|
| Kim1 | Forward | 5’-ACATATCGTGGAATCACAACGAC-3’ |
| Reverse | 5’-ACAAGCAGAAGATGGGCATTG-3’ | |
| Acta2 | Forward | 5’-ATCGTCCACCGCAAATGC-3’ |
| Reverse | 5’-AAGGAACTGGAGGCGCTG-3’ | |
| Col1a2 | Forward | 5’-TGCTGCTTGCAGTAACGTCG-3’ |
| Reverse | 5’-TCAACACCATCTCTGCCTCG-3’ | |
| Col3a1 | Forward | 5’-TGCCCACAGCCTTCTACACCT-3’ |
| Reverse | 5’-CAGCCATTCCTCCCACTCCAG-3’ | |
| Col4a1 | Forward | 5’-CTGGCACAAAAGGGACGAG-3’ |
| Reverse | 5’-ACGTGGCCGAGAATTTCACC-3’ | |
| Tnf | Forward | 5’-CATCTTCTCAAAATTCGAGTGACAA-3’ |
| Reverse | 5’-TGGGAGTAGACAAGGTACAACCC-3’ | |
| Il6 | Forward | 5’-GAGGATACCACTCCCAACAGACC-3’ |
| Reverse | 5’-AAGTGCATCATCGTTGTTCATACA-3’ | |
| Il10 | Forward | 5’-GCCTTCAGTATAAAAGGGGGACC-3’ |
| Reverse | 5’-GTGGGTGCAGTTATTGTCTTCCCG-3’ | |
| Tgfb1 | Forward | 5’-TGACGTCACTGGAGTTGTACGG-3’ |
| Reverse | 5’-GGTTCATGTCATGGATGGTGC-3’ | |
| Gapdh | Forward | 5’-GGCAAATTCAACGGCACAGT-3’ |
| Reverse | 5’-AGATGGTGATGGGCTTCCC-3’ |