Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Primers designed in this study to quantify the expression of ParCBF1, ParDAM5-6 and the reference gene.
| Primer | Sequence (5′ → 3′) | Annealing temperature (°C) | Amplicon length (bp) |
|---|---|---|---|
| qACT-F | GTGCCTGCCATGTATGTTGCCA | 60 | 226 |
| qACT-R | CAGTGGTGGTGAACATGTACCCYC | ||
| qCBF-F | GGCTACTTGAACTGGGATGACATG | 60 | 104 |
| qCBF-R | ACACAAACAAATACATGATTGAC | ||
| qDAM5-F | GCTTATGGATCCGGAGAGGCTGAATA | 60 | 101 |
| qDAM5-R | CAGCACTGTTGCAGGTGGTGGAGATA | ||
| qDAM6-F | GTTTGTGGAGCCGGAGACGTTGATT | 60 | 100 |
| qDAM6-R | GCAGCTGGTGGAGGTGGCAATTTGG |
