Figure 5.

Transmission-blocking efficacy of the AdHu5-Pf25-prime and AAV1-Pfs25-boost regimen. (A,B) Groups of BALB/c mice (n = 3) were immunized with the indicated regimen at a 6-week interval and infected with Pfs25DR3 at 35 days (A) or 287 days (B) after boost. Mosquitoes were allowed to feed on the infected mice for a DFA. At day 10–12 post-feeding, the mosquito midguts were dissected, and oocyst intensity and prevalence were determined (Table 2). Each datapoint represents a single mosquito blood-fed on each mouse. The x-axis points represent individual mice. Horizontal lines indicate the mean numbers of oocysts observed (± standard errors of the means [SEM]).